What with Hamburg-Lubeck, and HMA not all that long ago, it's a bit soon for another German route. But Austrian? That could be interesting.
If I had to guess: 1 French route (hopefully not a LGV) 2 American routes, a freight subdivision and a passenger route, or at least 1 US Passenger route. 1 old british route to see "steam in it's natural habitat" Possibly a route set outside GER/FR/UK/US and/or another german route (possibly set in the past, or featuring the ICE4/2/1 given the popularity of those suggestions and possible layering on existing routes)
Well, it's quite unlikely but I would love to see an Italian route. They have some cool high speed trains there (Italo or Frecciarossa), and like in LGV a beautiful scenery