No, there is not. You may watch the preview streams, they cover a lot of what you need to know bout the route and the how to drive.
As Rudolf notes, this is yet another DLC for which DTG has failed to provide a users' manual. However, the engine tutorials are fairly well done and worth running a few times before beginning to drive.
you can always read the train simulator classic manual of that route that gives you a good enough detail about things but yeah DTG seriously lacks in the readable manuals the last 2 years since they decided to just make some tutorial video's only nothing wrong with tutorial videos but if you wanna look up something fast it will be a pain at least some people in the community made some manuals themselves for others
I found and read this manual, but did not see the main one there - a description of the signals and signs
I literally downloaded stuff like this when I couldn't find a manual from DTG for the sim. Actually answered the questions I had. http://www.chartertoconductor.com/wp-content/uploads/2019/06/sd40a-om.pdf